TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| Alcaligenes faecalis | ||
| JQ135 | Strr; PA-degrading bacterium; Gram negative; wild type | CCTCC M 2015812 |
| Mut-H4 | Strr Kmr; picC (AFA_15145) mutant of A. faecalis JQ135 inserted by transposon | This study |
| JQ135ΔpicC | Strr; picC deletion mutant of JQ135 | This study |
| JQ135ΔpicC/pBBR-picC | Strr Gmr; JQ135ΔpicC complementation with pBBR-picC | This study |
| Escherichia coli | ||
| DH5α | F− recA1 endA1 thi-1 hsrdR17 supE44 relA1 deoRΔ(lacZYA-argF)U169 ϕ80lacZΔM15 | TaKaRa |
| BL21(DE3) | F− ompT hsdS(rB− mB−) gal dcm lacY1(DE3) | TaKaRa |
| SM10λpir | Donor strain for biparental mating | Lab stock |
| Plasmids | ||
| pET29a(+) | Kmr; expression plasmid | Novagen |
| pSC123 | Cmr Kmr; suicide plasmid, mariner transposon | Lab stock |
| pJQ200SK | Gmr mob+ orip15A lacZα+ sacB; suicide plasmid | Lab stock |
| pBBR1MCS-5 | Gmr; broad-host-range cloning plasmid | Lab stock |
| pJQ-ΔpicC | Gmr; picC gene deletion plasmid | This study |
| pBBR-picC | Gmr; the fragment containing the picC gene inserted into XhoI/HindIII-digested pBBR1MCS-5 | This study |
| pET-PicC | Kmr; NdeI-XhoI fragment containing picC gene inserted into pET29a(+) | This study |
| pET-PicCH12A | Kmr; NdeI-XhoI fragment containing picCH12A gene inserted into pET29a(+) | This study |
| pET-PicCH135A | Kmr; NdeI-XhoI fragment containing picCH135A gene inserted into pET29a(+) | This study |
| pET-PicCH163A | Kmr; NdeI-XhoI fragment containing picCH163A gene inserted into pET29a(+) | This study |
| pET-PicCH172A | Kmr; NdeI-XhoI fragment containing picCH172A gene inserted into pET29a(+) | This study |
| pET-PicCH177A | Kmr; NdeI-XhoI fragment containing picCH177A gene inserted into pET29a(+) | This study |
| pET-PicCH194A | Kmr; NdeI-XhoI fragment containing picCH194A gene inserted into pET29a(+) | This study |
| pET-PicCH216A | Kmr; NdeI-XhoI fragment containing picCH216A gene inserted into pET29a(+) | This study |