Fig. 3.

KLF12 as miR-137 target inhibits CSC phenotype in pancreatic cancer cells. a-b PCR and western blot analysis the KLF12 expression in miR-137-mimic and miR-137-inhibitor transfected AsPC-1 and PANC-1 cells. c Predicted binding sites of miR-137 in the wild type 3-UTRs of KLF12,Mutations in these 3-UTRs are highlighted in red. d Luciferase activity of reporters with wild type or mutant 3-UTR of KLF12 in the indicated cells co-transfected with the indicated oligonucleotides e KLF12 knockdown significantly impaired tumor-sphere formation in both in AsPC-1 and PANC-1 cells. f KLF12 knockdown decreased the CD133⁺ population in AsPC-1 and PANC-1 cells g Real-time PCR and western blot analyses showed that downregulation of KLF12 inhibited the expression of pluripotency-associated markers in AsPC-1 and PANC-1 cells