Fig. 3.
The senescence of cells in co-culture. a, b The staining for SA-β-Gal (green) in senescent BMSCs (a) and SnChos (b) in co-culture at 7 and 21 days. Bars = 100 μm. The ratio of SA-β-Gal positive cells was calculated (right, n = 3). c, d The expression level of p16Ink4a and p21Cip1 in BMSCs (c) and SnChos (d) in co-culture at 7 days and 21 days (n = 3). The MSCs (7d) or SnChos (7d) were used as control. *P < 0.05, **P < 0.01