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. 2019 Mar 12;10:86. doi: 10.1186/s13287-019-1193-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The senescence of cells in co-culture. a, b The staining for SA-β-Gal (green) in senescent BMSCs (a) and SnChos (b) in co-culture at 7 and 21 days. Bars = 100 μm. The ratio of SA-β-Gal positive cells was calculated (right, n = 3). c, d The expression level of p16^Ink4a and p21^Cip1 in BMSCs (c) and SnChos (d) in co-culture at 7 days and 21 days (n = 3). The MSCs (7d) or SnChos (7d) were used as control. *P < 0.05, **P < 0.01