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. 2019 Mar 12;20:205. doi: 10.1186/s12864-019-5554-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Correlation of differential gene expression and DNA methylation in roots and leaves show ROS association. Heatmap representation of differential gene expression (Log2 fold-change) and differential DNA methylation (average level in area indicated; Gene body: TSS to TTS, upstream: 2 kb from TSS, downstream: 2 kb from TTS) of 21 genes in roots (a) and 143 genes in leaves (b). Opposite trends are indicative of upregulated genes with hypomethylated DmCs or downregulated genes with hypermethylated DmCs whereas a consistent trend shows the converse relationship. ROS associated genes are indicated with an asterisk. c Venn diagram shows a visual representation of spaceflight DEGs and DmC-associated DEGs that overlap with ROS genes. d Venn diagram shows a visual representation of spaceflight DEGs and DmC-associated DEGs that overlap with housekeeping genes. e Visualization of the methylation data using the Integrative Genomics Viewer (IGV) (http://software.broadinstitute.org/software/igv/). The full–genome methylome profiling in the CHH context of spaceflight (Flight) and ground control (Ground) leaf sample along with DmC and DMR data in the corresponding region