HMGB1 levels in citrated plasma (<1.0 ng/mL, n = 24), EDTA-treated plasma (<1.0 ng/mL, n =
24), and serum (mean = 6.0 ± 2.1 ng/mL, n =
24) from healthy control subjects, determined by stable isotope dilution
IP 2D-nano-UHPLC-PRM/MS, and reported levels of serum HMGB1 determined
by ELISA in healthy control subjects (mean = 2.1 ± 0.9 ng/mL)
and cases (mean = 5.4 ± 2.8 ng/mL) for 18 of the studies shown
in Table 1 (indicated
with asterisks). Control plasma and serum samples from healthy subjects
for the LC/MS analyses were obtained from the same individuals. Data
are shown as means ± stndard deviation (SD).