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. Author manuscript; available in PMC: 2019 Mar 14.
Published in final edited form as: Cell Rep. 2019 Feb 5;26(6):1409–1418.e5. doi: 10.1016/j.celrep.2019.01.021

Figure 4. CD69+ Memory CD45iv− Lung Interstitial CD4+ T Cells Are Most Significantly Affected by HIV-1-Induced CD4+ T Cell Loss.

Figure 4.

HIV-1-infected humanized mice (n = 6) or uninfected control mice (n = 12) were labeled by injection of an anti-human CD45 antibody for 3 min prior to sacrifice. CD45iv− and positive CD4+ T cells were analyzed in the lungs, BAL, spleen, and blood by flow cytometry.

(A) Representative plots of CD45iv+ and CD45iv− CD4+ T cells in the lungs, BAL, spleen, and blood.

(B–D) The TRM-like phenotype of CD45iv+ or CD45iv− CD4+ T cells was further characterized by flow cytometry.

(B) Fold lung CD4+ T cell loss was analyzed by comparing CD4+ T cell numbers from infected with median uninfected control animals.

(C) The percentage of all CD4+ T cells with surface expression of CD69+ was analyzed by gating on viable CD4+ TCRα/β+CD45RO+CD62L−CD25− T cells.

(D) The percentage of all CD4+ T cells with surface expression of CCR5+ was analyzed by gating on viable CD4+ T cells.

(E and F) Correlation between intracellular HIV-1 p24+ CD4+ T cells 4 weeks after infection and number of (E) lung CD45iv− CD4+ T cell and (F) blood CD45iv+ CD4+ T cells.

The p values were measured by (A−D) Kruskal-Wallis and Dunn’s multiple comparisons tests and (E and F) Spearman test. Scatterplots are labeled with median and interquartile range. Each data point represents one humanized mouse sample.

See also Figures S3 and S4.