Fig. 1.

Analysis of the chromosomally integrated activation-induced promoters in CAR NK-92, CAR YT and T cells. a Scheme of the lentiviral vector (from 5’LTR to 3’LTR) encoding divergently transcribed copGFP and mCherry reporters driven by the constitutive EF1 promoter and one of the activation-inducible promoters, respectively. Organization of the activation-inducible promoters integrated into the genome as reporter constructs is shown below. b CopGFP-normalized mean fluorescence values of mCherry reporter in CAR NK-92 and CAR YT cells co-incubated with target HEK293T(PSMA) cells (blue) or isogenic controls (HEK293T, red). Similarly, the values observed for bead-activated (blue) and resting (red) T cells are shown. Mean fluorescence values are shown as circles and squares, with quartile ranges indicated by lines. Fold change of normalized mCherry signal is indicated next to each plot. AFU – arbitrary fluorescence units