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The nucleotide sequences of synthetic RBS with different translation initiation rates based on the prediction using RBS calculator (https://salislab.net/software/forward)
| Name | Strength | Predicted RBS sequence (5′–3′) |
|---|---|---|
| ZM4-Ptet-GFP-10 | 10 | CCATAATCTAGAGAAAGTAAGCAC |
| ZM4-Ptet-GFP-1000 | 1000 | AGGCTAAGAACTAACGGAGAGGTAAAT |
| ZM4-Ptet-GFP-10000 | 10,000 | ATCACAGGGTCTAGAAGGAGGTCGAA |
| ZM4-Ptet-GFP-Max | 15,000 | GAGCGAGAAGGAGGTAAAGT |
