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. 2019 Mar 12;15:88. doi: 10.1186/s12917-019-1833-9

Table 1.

Primers used to amplify the complete genome

Primers Sequences Position Size of PCR products (bp) Annealing temperature
P1-F CTCATTGGAGGGTTCGTTCG 1–20 191 58 °C
P1-R GCATGCGCGTGGTCAACCTAACA 169–191
P2-F CAAACGGGGAGGGCAAAATAAGA 186–208 1345 60 °C
P2-R GTGGTCGCAGGTCCGTAGAGC 1511–1531
P3-F GGGTGAAGAGAGAGTTCAACAA 1474–1497 1137 52 °C
P3-R CGTTACCAGGCCCAAGATAC 2591–2610
P4-F GGCATTTGAAAGCTGGAGCC 2473–2492 922 60 °C
P4-R GTCTTCGTCTGATCCTGCGT 3375–3395
P5-F TTGCGATTCCCAATGGATG 3089–3107 1171 58 °C
P5-R CCCAAATAGGTCCCTGTAGATA 4239–4260
P6-F CTACAACCCGGACCTGTGTC 3948–3967 921 60 °C
P6-R GCATGCGCGTGGTCAACCTAACA 4846–4868