Figure S1. Genotyping results for U2OS-GR clonal lines with an inserted promoter-proximal GBS (CGT).

(A) Genotyping of CGT insertion at the GYPC gene. Top: the sequence of the gRNA, the introduced GBS, and the efficiency of successfully edited single cell–derived clonal lines. Bottom: Sanger sequencing results for a successfully edited clonal line and the inferred sequence for each allele. (B) Genotyping of clones with a successfully added CGT GBS at the IL1B gene. (C) Genotyping of clones with a successfully added CGT GBS at the IL1R2 gene. (D) Genotyping of clones with a successfully added CGT GBS at the VSIG1 gene.