Figure S5. Induction of control genes for clonal lines edited at the GILZ GBS1.

(A) Relative mRNA expression levels as determined by qPCR for the unedited control GR target gene FKBP5 are shown for unedited parental U2OS-GR cells (wt), for unedited clonal control cell lines, and for clonal cell lines with GBS variants as indicated at the GILZ GBS1 locus. Averages ± SEM for cell lines treated overnight with 0.1 nM dex, 10 nM dex, or vehicle control (−) control are shown. Dots show the values for each individual clonal line. (A, B) Same as for (A) except that clonal lines as indicated were treated for either 0.5, 1, 2, or 4 h with 1 μM dex and expression of the unedited DUSP1 control gene is shown.