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. 2019 Mar 7;10:377. doi: 10.3389/fimmu.2019.00377

Figure 2.

Figure 2

OSM and OSMR expression and signaling in muscle cells. (A) Osm mRNA expression by qRT-PCR. Osm is expressed by all sorted infiltrating myeloid populations but absent in whole naïve skeletal muscle. Each dot represents an individual mouse (n = 3–4/group). Bars represent mean ± SD. (B) Osmr mRNA expression is only present in sorted mouse muscle satellite and interstitial cells, not in any sorted myeloid population infiltrating the injured muscle (C) Phos-flow of pSTAT3 Y705 phosphorylation in cultured CD45−Ter119−CD31−CD34+Sca1+ muscle interstitial cells (IC), CD45−Ter119−CD31−CD34+Sca1− muscle satellite cells (SC), and Kusa4b10 cells. Cells were incubated for 10 min at 37°C with medium alone (unstimulated), or 25 ng/ml recombinant mouse OSM plus DMSO or of 25 ng/ml OSM plus 1 μM ruxolitinib. Cell were then fixed and permeabilized before staining with AlexaFluor647-conjugated mouse mAb anti-pSTAT3 (Y705) and analyzed by flow cytometry. Representative figures were one of two independent experiments.