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. 2019 Mar 7;10:377. doi: 10.3389/fimmu.2019.00377

Figure 3.

Figure 3

Persistence of STAT3 tyrosine phosphorylation in injured muscles following spinal cord injury. Western-blots of whole muscle lysates collected from mice that underwent either SCI or SHAM surgery together with an intramuscular injection of either CDTX or PBS. Western blot of whole muscle lysates from individual mice taken on day 4 (A), 7 (B), or 14 (C) post-injury. Each membrane was probed with rabbit anti-pSTAT3 Y705 mAb, stripped and then re-probed with rabbit anti-total STAT3 mAb. Band fluorescence intensity was quantified and ratio of signal intensity of pSTAT3 vs. total STAT3 calculated for each individual mouse and normalized relative to the average pSTAT3/STAT3 ratio in control mice (SHAM+PBS) at each time-point. Each lane and each dot represents a separate mouse, n = 2–4 mice/treatment/time-point. Bars represent mean ± SD. P values were calculated by ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.