Skip to main content
. Author manuscript; available in PMC: 2019 Mar 14.
Published in final edited form as: Adv Exp Med Biol. 2018;1074:585–591. doi: 10.1007/978-3-319-75402-4_71

Fig. XX.1. Interactions of Akt2 with POSH in the yeast two-hybrid assay.

Fig. XX.1

Yeast plasmid of pLexA-ΔPH-Akt1, pLexA-ΔPH-Akt2, or pLexA-lamin and VP16-POSH was used to simultaneously transform the L40 yeast strain that harbors both His and LacZ reporters under the control of LexA binding sites. Transformants were plated on media lacking tryptophan and leucine (A), or tryptophan, leucine, and histidine plus 5 mM aminotriazole (B). Cells surviving on these plates were further screened for the LacZ phenotype by filter assay (C, D). Lifted colonies were scored for LacZ phenotypes by detection of a blue color in the presence of 5-bromo-4-chloro-3-indolyl β-D-galactosidase after incubation at room temperature for 2-to-4 h.