Skip to main content
. Author manuscript; available in PMC: 2019 Mar 14.
Published in final edited form as: Adv Exp Med Biol. 2018;1074:585–591. doi: 10.1007/978-3-319-75402-4_71

Fig. XX.2. GST pull-down experiments.

Fig. XX.2

Individual HA-tagged Akt isoforms were expressed in HEK-293T mammalian cells. Expressed proteins were incubated with GST or GST-POSH, and then GST pull-down assays were carried out. The bound proteins were washed and subjected to immunoblot analysis with anti-HA (A) and anti-GST (B, C) antibodies.