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. 2018 Sep 8;123(4):579–585. doi: 10.1093/aob/mcy168

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Fig. 2. — Immunolocalization of rhamnogalacturonan-I pectin epitopes in guard cell walls of Arabidopsis. Black round dots in images are colloidal gold labels attached to the specific pectin antibody. (A) LM6 antibody strongly localizes in guard cell walls, especially in the interior, but not towards the ledges (arrowhead). (B) Sparse labelling of the LM13 antibody (arrows) towards the interior of the guard cell outer wall. (C) Guard cell walls label weakly with LM13 antibody. (D) LM6 antibody recognizes the thick interior of guard cell (gc) walls and is absent in epidermal cell walls. (E) Labelling with LM5 antibody is absent in guard cell (gc) walls and low in epidermal cell walls (arrow). Scale bars = 500 nm.