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Immunofluorescence microscopy imaging of S100A8 expression in the skin of LD patients and controls. Skin tissue sections from the EM rash site of three individual LD patients and one control patient (Control 1) were subjected to immunostaining for S100A8 (green) and nuclei staining with DAPI (blue) before being subjected to fluorescence microscopy at 20× magnification as described in Experimental. Dotted lines separate the epidermis from the dermis. Bar represents 50 μm.
