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. 2019 Mar 14;14(3):e0213929. doi: 10.1371/journal.pone.0213929

Table 3. Costs per test according to number of samples.

Test Stages Category Sample Quantity per Test
1 2 3 4 5 6
DNA Extraction (3.93 hours) Manpower $38.98 $19.49 $12.99 $9.74 $7.80 $6.50
Reagentsa $1.46 $1.46 $1.46 $1.46 $1.46 $1.46
Real Time PCR
(2.33 hours)
Manpower $23.12 $11.56 $7.71 $5.78 $4.62 $3.85
Materiala $1.07 $0.64 $0.50 $0.43 $0.39 $0.36
Reagentsa $8.56 $5.13 $3.99 $3.42 $3.08 $2.85
Data Reporting
(0.33 hours)
Manpower $3.30 $1.65 $1.10 $0.83 $0.66 $0.55
Total cost per test $76.50 $39.94 $27.76 $21.67 $18.01 $15.57

Detailed costs regarding DNA extraction, real time PCR and data reporting considering samples per test. The time expected for each stage is also described.

a Material and reagents costs were calculated with an addition of 10% expected losses and four controls.