Figure 8. Determination of dissociation constants from tracer diffusion and surface density.

Binding was simulated with a binding probability p_bind = 0.1 and three different average binding times of τ₁ = 0.025ms (Δ), τ₂ = 0.1ms (○), τ₃ = 0.2ms (□), which correspond to dissociation constants $\overline{K_{D,1}}\approx 0.2,\overline{K_{D,2}}\approx 0.05\text{and}\overline{K_{D,3}}\approx 0.03,$ for τ₁, τ₂ and τ₃, respectively. Mobility ratios D_ON/D_OFF at t_lag = 200ms (A) or ρ_ON/ρ_OFF (B) are plotted as a function of area fraction covered by obstacles. The fits (gray lines; models described in the main text) are in good agreement with simulated data; fit results are provided in the figures.