Fig. 2.

Fat-specific protein (FSP27) protects against growth hormone (GH)-induced lipolysis and improves insulin signaling in human adipocytes. A: qPCR analysis of FSP27 and G0S2 mRNA from differentiated human adipocytes treated with 250 ng/ml GH for 2 h. Data are shown as mean ± SE of 3 independent experiments. B: lipolysis (glycerol release) from human adipocytes treated with GH for 2 h in the presence of control adenovirus (expressing CFP only) or adenonovirus expressing FSP27. C: Nile red staining showing lipid droplet size in control and GH-stimulated differentiated human adipocytes. Lipid droplets show significant reduction in size upon GH treatment. D: Western blot showing impaired AKT signaling (pAKT Ser⁴⁷³) and total AKT in primary human adipocytes. Ctr, control. Data are shown as means ± SE of 3 independent experiments. *P < 0.05 and ***P < 0.001.