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. 2019 Mar 14;14(3):e0213830. doi: 10.1371/journal.pone.0213830

Fig 1.

Fig 1

(A) Bovine ELISA close to cut-off confirmation via WB assay. Lane 1: PageRuler™ Pre-stained Protein Ladder (Thermo Fisher Scientific, USA); Lane 2: rTES–30 with pooled sera from four seropositive animals; Lane 3: rTES–120 with pooled sera from four seropositive animals; Lane 4: rTES–30 with pooled sera from four seronegative animals; Lane 5: rTES–120 with pooled sera from four seronegative animals. (B) Horse ELISA close to cut-off, confirmed by the WB assay. Lane 1: rTES–30 with pooled sera from four seropositive animals; Lane 2: rTES–120 with pooled sera from four seropositive animals; Lane 3: rTES–30 with pooled sera from four seronegative animals; Lane 4: rTES–120 with sera from four seronegative animals; (C) Sheep ELISA close to cut-off, confirmed by the WB assay. Lane 1: rTES–30 with pooled sera from four seropositive animals; Lane 2: rTES–120 with pooled sera from four seropositive animals; Lane 3: rTES–30 with pooled sera from four seronegative animals; Lane 4: rTES–120 with pooled sera from four seronegative animals.