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. 2019 Mar 14;14(3):e0213932. doi: 10.1371/journal.pone.0213932

Fig 1. Kinome siRNA screening for cytotoxic effects of ARPE-19 cells during oxidative stress.

Fig 1

(A) Human RPE ARPE-19 cells were treated with non-targeting siRNA for 48 h, followed by treatment with hydrogen peroxide at 62.5, 125, 250, 500, and 1000 μM for 24 h in order to determine cell viability. Cellular ROS production and (B) cell viability were measured with ROS-Glo and Cell-titer Glo, respectively. (C) Cells were treated with kinome siRNA (710 gene) for 48 h followed by treatment with hydrogen peroxide (500 μM) for 24 h in order to measure ROS production in cells. (D) The top 10-ranked hits from kinome siRNA screening were further validated for cellular ROS production in three independent experiments (Three parallel samples were included in each experiment), and the results are shown as mean ± SEM.