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. 2019 Mar 14;14(3):e0213932. doi: 10.1371/journal.pone.0213932

Fig 2. Effects of ERBB2 on autophagy in ARPE-19 cells during oxidative stress.

Fig 2

(A) Human RPE ARPE-19 cells were transfected with 5 nM scramble siRNA or siRNA against ERBB2 for 64 h and then treated with hydrogen peroxide (500 μM) for 8 hr. The cells were lysed for western blotting using antibodies against NRF2, SQSTM1, MAP1LC3B, ATG4B, and ACTB. (B) The quantitative results for ratio of MAP1LC3B-II/I and SQSTM1 protein level are shown. (C) The mRNA levels of SQSTM1 in cells as mentioned above were determined by real-time polymerase chain reaction (PCR). The results were analyzed using Prism 5.0 and expressed as mean ± SEM from three independent experiments (Three parallel samples were included in each experiment).