Fig 1. Effects of MOI on adhesion and invasion of Ca9-22 cells by P. gulae D049.
Antibiotic protection invasion assays with P. gulae D049. Ca9-22 cells were infected with P. gulae at an MOI of 1, 10, 100, or 1000 for 90 min. The numbers of adherent and/or intracellular bacteria were determined by counting viable cell lysates, and are expressed as percentage of input bacterial cell number. Values are shown as the mean ± SD of three independent experiments and were analyzed with a t test. *P <0.01 (Student’s t test), as compared with infected cells (MOI 1, 10, and/or 1000). A; Adhesion efficiency, B; Invasion efficiency.