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. 2019 Mar 14;9(3):035221. doi: 10.1063/1.5084261

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© 2019 Author(s).

2158-3226/2019/9(3)/035221/6/$0.00

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FIG. 1. — Schematic depiction of the magnetic pulling cytometer set-up (left), showing the 3D printed adapter, permanent neodymium magnets, 416 stainless steel probe, and the ‘magnetic bead on gel’ arrangement with a 4.5 μm superparamagnetic bead bound to a polyacrylamide (PAA) gel attached to a glass coverslip. Insets on the right show the magnetic bead coated in protein A (green) atop the PAA gel coated with IgG (red). Fluorescent beads are embedded within the gel to track the force induced displacements in the gel. Protein A binding to the IgG facilitates magnetic bead binding to the PAA gel in the ‘magnetic bead on gel’ arrangement.