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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: Nature. 2019 Mar 6;567(7747):267–272. doi: 10.1038/s41586-019-1002-0

Fig. 3 |. TRAIP promotes NEIL3-dependent ICL repair.

Fig. 3 |

a, pICLAP was replicated in the indicated extracts and analyzed as in Fig. 1b. Red dot, OC-OC catenane; Blue dot, OC-SC catenane. For TRAIP levels in extracts, see Extended Data Fig. 6c.

b, Error-free repair of pICLAP was quantified as in Fig. 1f. Error bars, standard error of the mean from three independent experiments. For TRAIP levels in extracts, see Extended Data Fig. 6d.

c and f, Analysis of proteins associated with pICLPt during replication in the indicated extracts (c) or undepleted extract (f) in the presence of p97i and Culi. Asterisk, non-specifically detected protein.

d, Schematic of Xenopus NEIL3 proteins.

e, pICLAP was replicated in the indicated extracts and analyzed as in Fig. 1b. ICL unhooking was estimated by quantifying OC and SC signal normalized to pQnt. For NEIL3 levels in extracts, see Extended Data Fig. 8c.