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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Mol Microbiol. 2019 Jan 24;111(3):811–824. doi: 10.1111/mmi.14193

Figure 3. DAP-R independent of LiaR does not result in phospholipid microdomain redistribution or a reduction in DAP binding.

Figure 3.

(A) N-nonyl acridine orange (NAO) and BODIPY-DAP staining of E. faecalis strains. NAO is a membrane dye that associates with anionic phospholipids, particularly cardiolipin. In all strains NAO and BODIPY-DAP can be seen localizing to the division septum (white arrows), without any areas of redistribution. (B) Quantitative BODIPY-DAP binding. Comparisons in binding were made between parental stains (bold) and derivatives. Increased DAP binding was seen in OG1RFΔliaRyxdK202dak371ace at both 4 and 64 µg/mL as compared to OG1RFΔliaR. No significant changes were seen between the other strain pairs. Representative results of two independent runs performed on different days.