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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Mol Microbiol. 2019 Jan 24;111(3):811–824. doi: 10.1111/mmi.14193

Figure 4. Mutations in DAK and yxdJ alter membrane fluidity.

Figure 4.

Membrane fluidity was assayed using the fluorescent dye laurdan, whose emission spectrum shifts in response to the order of the lipid bilayer (higher numbers indicate increasing rigidity). Representative results are displayed as the mean and standard deviation of two independent experiments run in technical quadruplicate, with DAP or ethanol added at time=0. (A) and (B) show that baseline fluidity and fluidity after the addition of ethanol are similar for both OG1RF and OG1RFΔliaR. (C) and (D) As compared to OG1RF wild type, mutants with changes in DAK had a significantly more rigid membrane at baseline, and were more resistant to the fluidity shift after addition of ethanol. (E) Complementation with the wild type dak allele restored membrane fluidity to wild type levels. (F) Comparison of mean baseline fluidity by two-way ANOVA with correction using Dunnett’s multiple comparisons test, using OG1RF as the control strain for comparison. ***p<0.001; ns, non-significant. Representative results of two independent assays for each strain performed on different days.