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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Anesthesiology. 2019 Apr;130(4):592–608. doi: 10.1097/ALN.0000000000002615

Fig.3. TRPV1 agonist capsaicin induces a stronger potentiation of glutamate transmission and firing in LHb neurons from ethanol naive rats.

Fig.3.

Representative traces showing enhancement of sEPSCs induced by 3 µM capsaicin in LHb neurons from a Naive (A1) or an EtOH-WD (B1) rat. (A2-B2) Exemplar current traces were acquired in A1 and B1, before and during capsaicin application. Cumulative probability plots show higher incidence of events with shorter inter-event interval, and amplitude before and after capsaicin application in the LHb neurons of Naive (A3) and EtOH-WD (B3) rats (k-s test). Capsaicin elicited a concentration-dependent increase in sEPSC frequency (C), which was significantly greater in Naïve than in EtOH-WD neurons. The smooth curve is the best fit to the data by the logistic equation. Capsaicin did not significantly alter the mean sEPSC amplitude (D). (E) A representative example of increased spontaneous firing rate induced by 3 µM capsaicin in a Naïve or an EtOH-WD neuron. (F) Capsaicin caused a concentration-dependent increase in firing rate, which was significantly greater in the Naïve than the EtOH-WD neurons. *P < 0.05, **P < 0.01, ***P < 0.001 relative to 0.01 μM capsaicin; ###P < 0.001, Naïve in comparison with EtOH-WD rats. ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, Naïve in comparison with EtOH-WD rats underwent the same dose of capsaicin. Data were analyzed with two-way ANOVA and Tukey post hoc comparison.