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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Mol Microbiol. 2018 Dec 12;111(3):570–587. doi: 10.1111/mmi.14174

Figure 9. ArcA regulates SPI1 expression independent of ArcZ.

Figure 9.

β-galactosidase activity in Salmonella strains containing the (A) hilD’-’lacZ translational fusion or the (B) hilA’-lacZ transcriptional fusion in a wild type, ΔarcZ, and/or ΔarcA background grown in high aeration conditions. (C) β-galactosidase activity in Salmonella strains containing hilA’-lacZ+ transcriptional fusion and the indicated mutations in the spi1 (Δspi1 ΔrtsA) background with RtsA protein produced under a tetracycline regulated promoter grown under high aeration conditions with the indicated a-tetracycline concentrations. β-galactosidase activity units are defined as (μmol of ONP formed min−1) × 106/(OD600 × ml of cell suspension) and are reported as mean ± standard deviation where n=3. Strains used: JS892, JS2124, JS2143, JS2144, JS749, JS2126, JS2145, JS2146, JS2147, JS2148, JS2149, JS2150, JS2077, JS2151, JS2152, and JS2153.