Table 2.
Primers used in this study
| Primer name | Sequence (5’ -> 3’) |
|---|---|
| Deletion alleles | |
| ΔcysB | |
| ΔcysB-5-SalI-u | GGGTCGACGGATCAGCGTGGTGTGCCTTATCA |
| ΔcysB-5-XbaI-l | GCTCTAGACTCAGCAGTCGATGATACATTCAA |
| ΔcysB-3-XbaI-u | GCTCTAGATAAGATCGCCCTGATATTATTTTC |
| ΔcysB-3-SacI-l | GGGAGCTCGGTATGTTCTCTTTCTCTGGTCTT |
| ΔtcyJ | |
| ΔtcyJ-5-XbaI-u | GGTCTAGAGTTCCTTGTTCGACGATCTTTCCA |
| ΔtcyJ-5-XmaI-l | GGCCCGGGGATTACTCACCATTCATCAAATAA |
| ΔtcyJ-3-XmaI-u | GGCCCGGGGTATTAGGATGATACTTTTTTTGC |
| ΔtcyJ-3-EcoRV-l | GGGATATCGCATTCGCAATAACACGATTTAAT |
| ΔtcypP | |
| ΔtcypP-5-SalI-u | GGGTCGACGATTACCTAACACCCAAATATCAT |
| ΔtcypP-5-HindIII-l | CCGAAGCTTTACGTTGCTGTGTATTAATAAAAAAG |
| ΔtcypP-3-HindIII-u | GGTAGAAAGCGCACAAGCTTAAT |
| ΔtcypP-3-SacI-l | GGGAGCTCGTGCTATACGAGATGTTATTACTC |
| cysB alleles | |
| cysB+ | |
| cysB+-KpnI-u | GGGGTACCGTAGTAATATTTTCCAGTGTAACA |
| cysB+-SpeI-l | GGACTAGTGGTCTATATTTAAAAACTGCTGAG |
| cysBA227D | |
| cysBA227D-u | GTCTTTACCGCAACCGATGATGATGTCATTAAGACTTATG |
| cysBA227D-l | CATAAGTCTTAATGACATCATCATCGGTTGCGGTAAAGAC |
| promoter reporters | |
| PtcyJ-XmaI-u | GGCCCGGGGAAGCCAAAGCGATGAAGACACGG |
| PtcyJ-XbaI-l | GGTCTAGAGCGAGGATATTCTTCGTCCATTTC |
| PcysD-XmaI-u | GGCCCGGGAGAGGTTCATAGTGTGTCTCCAAA |
| PcysD-XbaI-l | GGTCTAGATTGCTGAAGGTGGGTCAATCGTTT |
| PcysJ-XmaI-u | GGCCCGGGGGTAAGAATCAACGAATAAACGAT |
| PcysJ-XbaI-l | GGTCTAGAGAGAGTTCCTTTAATAACATGACG |
| PcysK-XmaI-u | GGCCCGGGGATGTTTACCGTGATACACATAAA |
| PcysK-XbaI-l | GGTCTAGAGAGAATTATCTTCGTAAATCTTTG |
| PtetA-XmaI-XbaI-u | CCGGGTTGACACTCTATCATTGATAGAGTTATTTTACCACTCGCT |
| PtetA-XmaI-XbaI-l | CTAGAGCGAGTGGTAAAATAACTCTATCAATGATAGAGTGTCAACTC |