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. 2019 Mar 8;10:429. doi: 10.3389/fimmu.2019.00429

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Copyright © 2019 Luo, Li, Zhou, Lv, Wu, Chen, Zhang, Sui, Tu, Cui, Chen, Fu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR7 promotes the generation of PCs and ASCs. TLR7^−/− and WT mice were immunized i.m. with 10⁶ FFU LBNSE in the hind legs. (A,B) Bone marrows were collected at the indicated times p.i. Total 10⁵ events were acquired for analysis of plasma cells (B220^loCD138⁺) (n = 5–6). (C,D) Draining lymph nodes were collected at 7 d.p.i. for analysis of RABV-specific ASCs by ELISpot (n = 3). Representative sections are shown in (C) and numbers of ASCs are shown in (D). Error bars represent SEM (^**P < 0.01; ^***P < 0.001).