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. 2019 Mar 8;10:118. doi: 10.3389/fendo.2019.00118

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Copyright © 2019 Chen, Li, Wang, Song, Li, Xie, Li, Chen, Ma, Chen, Lv, Li and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The kinase and phosphorylated kinase protein levels of the testis of rats with or without FGF1 treatment in vivo. (A) gel; (B-D) quantitative data. The protein levels of pAKT1, AKT1, pAKT2, AKT2, pERK1/2, ERK1/2, and ACTB (control) were analyzed by Western blot in the testes from rats treated with 0, 10, and 100 ng/testis FGF1 on post-EDS day 14 for 14 days. Values include the ACTB-adjusted levels of pAKT1, AKT1, pAKT2, AKT2, pERK1/2, and ERK1/2 as well as the ratios of pAKT1/AKT1, pAKT2/AKT2, and pERK/ERK. Mean ± SEM, n = 4–5. Asterisk (^*) designates significant difference at P < 0.05 when compared to the control (0 ng/testis FGF1).