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. 2019 Mar 8;10:118. doi: 10.3389/fendo.2019.00118

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Copyright © 2019 Chen, Li, Wang, Song, Li, Xie, Li, Chen, Ma, Chen, Lv, Li and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EdU incorporation into stem Leydig cells and indirect measurement of medium testosterone (T) levels after FGF1 treatment in vitro. Immunofluorescent staining of EdU (green color in the nucleus) of stem Leydig cells on the surface of the seminiferous tubules treated with 0 (A), 10 (B), and 100 ng/ml FGF1 (C) for 7 days; (D): quantitative data of percentage of EdU-positive cells per cm², Mean ± SEM, n = 3. White arrow designates EdU-positive stem Leydig cells. Bar = 50 μm. (E,F), medium testosterone (T) levels after the seminiferous tubules were treated initially by FGF1 and/or 1 μM PD173074 (PD) and/or 1 μg/ml heparin (HA) for 7 days and then the tubules were switched in LCDM for additional 2 weeks. Mean ± SEM, n = 6. Identical letters designate no significant differences at P < 0.05 between two groups.