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. 2019 Mar 8;10:402. doi: 10.3389/fmicb.2019.00402

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Copyright © 2019 Ryder, Sandford, Manners, Dalton, Wiles and Kirman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization with BCG enhanced resident populations of CD11b^high macrophages. Differences in (A) frequency and (B) number of CD11b^high alveolar (CD11c^high; top) and interstitial (CD11c^int; bottom) macrophages, and (C) their proportion within the tdTomato+ compartment at 1 or 14 days post-infection in mice given BCG tdTomato or vehicle control (–) i.n. Medians shown for n = 7–8 mice per group, data represent two independent experiments, n = 7–8. Mann–Whitney U-test for differences between naïve and immunized groups: ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001.