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. 2019 Mar 8;10:204. doi: 10.3389/fphys.2019.00204

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Copyright © 2019 Bonelli, Bruno, Caccia, Sgambetterra, Cappellozza, Jucker, Tettamanti and Casartelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Main cell types present in the larval midgut epithelium. (A) Columnar cells display a developed basal infolding (arrows) and long microvilli (mv). (B) Endocrine cells with electron-dense granules (g) inside the cytoplasm. (C) Regenerative cells (arrowhead) located at the base of the midgut epithelium. (D,E) The number of regenerative cells (arrowheads) increases during larva-larva molt (D) compared to the last larval instar, when the insect is actively feeding (E). (F) Western blot analysis of phospho-Histone 3 (H3P). e: epithelium; l: lumen; n: nucleus. Bars: 5 μm (A), 2 μm (B,C), 50 μm (D), 20 μm (E).