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. 2019 Mar 8;10:204. doi: 10.3389/fphys.2019.00204

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Copyright © 2019 Bonelli, Bruno, Caccia, Sgambetterra, Cappellozza, Jucker, Tettamanti and Casartelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological organization of MMG1. (A,B) Copper cells (c) characterized by the long microvilli (mv). (C,D) Elongated mitochondria (arrowheads) are visible in longitudinal (C) and cross (D) section of microvilli (mv). (E) Portasome-like structure (arrow) in the apical surface of microvilli. Mitochondria (arrowheads) are visible. (F–J) H⁺ V-ATPase immunolocalization (white arrowheads) in the different midgut regions. (K) Western blot analysis of H⁺ V-ATPase. e: epithelium; l: lumen; n: nucleus; pm: peritrophic matrix. Bars: 20 μm (A,G–K), 10 μm (B), 1 μm (C,D), 200 nm (E).