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. 2019 Mar 8;10:204. doi: 10.3389/fphys.2019.00204

FIGURE 9.

FIGURE 9

Expression profile of serine proteases. (A,B) qRT-PCR analysis of HiTrypsin (A) and HiChymotrypsin (B) in the different midgut regions. The values are reported as relative expression mean ± SEM of 3 experiments. Different letters denote significant differences: HiTrypsin (ANOVA test followed by Tukey’s test. ANOVA p-value < 0.001, Tukey’s test p-values: AMG vs. MMG1 p < 0.001, AMG vs. MMG2 p = 0.107, AMG vs. PMG1 p < 0.001, AMG vs. PMG2 p < 0.001, MMG1 vs. MMG2 p < 0.05, MMG1 vs. PMG1 p < 0.001, MMG1 vs. PMG2 p = 0.328, MMG2 vs. PMG1 p < 0.001, MMG2 vs. PMG2 p < 0.001, PMG1 vs. PMG2 p < 0.001); HiChymotrypsin (ANOVA test followed by Tukey’s test. ANOVA p-value < 0.001, Tukey’s test p-values: AMG vs. MMG1 p < 0.01, AMG vs. MMG2 p = 0.237, AMG vs. PMG1 p < 0.001, AMG vs. PMG2 p < 0.001, MMG1 vs. MMG2 p < 0.001, MMG1 vs. PMG1 p < 0.01, MMG1 vs. PMG2 p < 0.01, MMG2 vs. PMG1 p < 0.001, MMG2 vs. PMG2 p < 0.001, and PMG1 vs. PMG2 p < 0.790).