Table 1.
Statistics for the solution structure of METTL3 ZFD
| Number of constraints | |
| NOE | 857 |
| Intra (i = j) | 556 |
| Short range (|i-j| < 3) | 205 |
| Long range (|i-j| ≥ 3) | 96 |
| PRE | 52 |
| H-bond | 32 |
| Dihedral angles | 156 |
| φ | 65 |
| ψ | 60 |
| χ1 | 31 |
| SAXS constraints# | 2 |
| Zn2+-coordination constraints¶ | 32 |
| Structure statistics | |
| Number of violations (per structure) | |
| NOE derived constraints (>0.5 Å) | 0 |
| PRE derived constraints (>5.0 s−1) | 0.63 |
| Dihedral angle constraints (>5°) | 0 |
| RMS of constraints | |
| NOE derived constraints (Å) | 0.09 ± 0.002 |
| PRE derived constraints (s−1) | 1.81 ± 0.05 |
| PRE Q-factor | 0.09 ± 0.002 |
| Dihedral angle constraints (°) | 0.68 ± 0.07 |
| SAXS constraints | 0.04 ± 0.002 |
| Average Pairwise r.m.s. deviation (Å) | |
| Backbone heavy atoms§ | 0.90 ± 0.20 (all) |
| 0.50 + 0.09 (core) | |
| All heavy atoms | 1.22 ± 0.16 (all) |
| 0.95 ± 0.07 (core) | |
| Ramachandran plot appearance | |
| Most favored | 65.8% |
| Additionally allowed | 26.0% |
| Generously allowed | 6.3% |
| Disallowed | 1.8% |
#Collected for ZFD constructs spanning residues 259–357 and residues 259–338
¶Distance and dihedral angle restraints for the two CCCH-type Zn2+-finger (Peters et al. 2010)
§The core rigid residues of the ZFD comprise residues 276–336