Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 14;9:4605. doi: 10.1038/s41598-019-40810-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Transactivation of Acan enhancers by PAX1/PAX9 and SOX9/SOX5/SOX6. (a–f) Dual luciferase assays were performed with HEK293T cells (a–c), chondrocytes (d,e), or NIH3T3 cells (f). Cells were co-transfected with reporter plasmids (pGL4.74 [hRluc/TK] and pGL4.23[luc2/minP] or pGL3-Basic vectors) and expression plasmids (pcDNA3 vectors). The pGL4.23[luc2/minP] and pGL3-Basic vectors used for the assays were pGL4.23[luc2/minP]-1xUE-Luc (1xUE-Luc), pGL4.23[luc2/minP]-4xUE-Luc (4xUE-Luc), or pGL4.23[luc2/minP]-I12E-Luc (I12E-Luc), and pGL3-Basic-Nkx3.2-P-Luc (Nkx3.2-P-Luc). The expression plasmids used were pcDNA3 empty vector (pcDNA3), pcDNA3-hSox5 (Sox5), pcDNA3-hSox6 (Sox6), pcDNA3-mSox9 (Sox9), pcDNA3-FLAG-Pax1 (Pax1), or pcDNA3-FLAG-Pax9 (Pax9). For the co-transfection of pcDNA3 vectors in (b), 10 ng each of Sox5, Sox6, and Sox9, and 20 ng of pcDNA3, Pax1, or Pax9 were used. For the co-transfection of pcDNA3 vectors in (f), 25 ng each of Pax1 and Pax9 (the fourth bar from the left), or 25 ng of Sox9 and 25 ng of pcDNA3, Pax1, or Pax9 (from the fifth to the seventh bars from the left), or 12.5 ng each of Pax1 and Pax9 (the right end bar) were used. The firefly and Renilla luciferase activities were measured 24 h after transfection. Values were normalized using a pGL4.74[hRluc/TK] vector and are presented as fold induction relative to pcDNA3 co-transfected with reporter plasmids. Graphs show a representative experiment out of at least three. Each bar represents the average of three independent transfections (means ± s.d.). *P < 0.05 versus pcDNA3, **P < 0.01 versus pcDNA3, ***P < 0.001 versus pcDNA3 (a,c,d,e,f), pcDNA3 + Sox5 + Sox6 + Sox9 (b), or pcDNA3 + Sox9 (f).