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. 2019 Mar 14;9:4450. doi: 10.1038/s41598-019-41023-5

Figure 5.

Figure 5

Super-resolution microscopy analysis shows that M2e-specific IgGs can disrupt pre-existing filaments (n = 50). MDCK cells were seeded in 8 well microslides, treated with M2e-speficic MAb 37 (IgG1), MAb 65 (IgG2a), MAb 148 (IgG1), or isotype control IgG1 + IgG2a at 20 μg/mL at 24 h post infection with A/Udorn/72 at MOI 5 in serum-free medium. The infected cells were incubated with M2e-specific MAbs for 1 h at 37 °C. The cells were then washed with PBS and fixed with 2% PFA at room temperature for 20 min. infected cells and A/Udorn/72 filaments were visualized by immune-staining with polyclonal convalescent mouse serum directed against A/Udorn/72, followed by Alexa Fluor 647 Donkey Anti-Mouse IgG serum. (a) STORM images showing disruption pre-existing filament when MDCK cells are cells treated with M2e-speficic MAbs at 24 h post infection. (b) Representation of the length (µm) of all filaments quantified (n = 50) from infected cells and treated with MAb. Bar = 5 µm. The experiments were performed in triplicate wells for each condition and repeated at least three times with similar results. One-way ANOVA with multiple comparisons correction (Kruskal–Wallis test). ***p ≤ 0.001; ****p ≤ 0.0001.