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. 2019 Mar 14;9:4572. doi: 10.1038/s41598-019-41040-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTGS2 inhibition by NS-398 in transgenic astrocyte-like cells does not alter their capability to stimulate microglial activation. (A) mNPsc-derived astrocytes overexpressing FUS and treated for 24 hrs with 10 ng/ml IL1β in the presence of the selective PTGS2 inhibitor NS-398 (10 μM), produced negligible amounts of PGE₂, compared to non-inhibited cells, as assessed by EIA analysis. Data are means ± SEM, n = 3; *P < 0.05. (B) Nitrite levels in the medium of microglia exposed to CMs from FUS overexpressing cells treated with 10 ng/ml IL1β in the presence of NS-398 (CM + Dox + IL1β + NS-398) were comparable to the levels induced by the CMs from non-inhibited astrocytes (CM + Dox + IL1β). Data are expressed as the fold increase versus nitrite levels produced by microglia exposed to CMs from non transgenic astrocytes (CM − Dox + IL1β), and are means ± SEM, n = 3.