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. 2019 Mar 14;9:4490. doi: 10.1038/s41598-019-41016-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Androgens induce AR/Src/PI3-K complex assembly and FAK activation in MDA-MB453 cells. Quiescent MDA-MB453 cells were used. In (a–c), cells were stimulated for the indicated times with 10 nM R1881. In (a), lysate proteins (5 mg/ml) were immune-precipitated using the anti Src antibody (Src) or control IgG (IgG mouse). Proteins in immune-complexes were analyzed by Western blot, using the antibodies against the indicated proteins. In (b,c), lysate proteins were analyzed by Western blot, using the antibodies against the indicated proteins. P-Fak stands for P-Tyr397 Fak. The blot in (c) is representative of two different experiments. The filters were stripped and re-probed using anti tubulin antibody, as loading control.