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. 2019 Mar 14;9:4489. doi: 10.1038/s41598-019-41119-y

Figure 5.

Figure 5

Colocalized 4-plex staining of ER (AF647), CK (AF546), PR (AF488) and Her2 (AF350) applied to the breast TMA. 4-plex staining performed on the same cores used for the antibody removal characterization in Fig. 2. Specific staining is observed for each marker. The left column shows the overview of the TMA cores, scale bar 500 μm. Columns 2–6: (A) Core employed for the antibody removal characterization of ER (Fig. 2A), expressing also PR and CK staining and negative to Her2. (B) Core employed for the antibody removal characterization of CK (Fig. 2B), expressing also ER and PR, and negative to Her2. (C) Core employed for the antibody removal characterization of PR (Fig. 2C), expressing also CK and negative to ER and Her2. (D) Core employed for the antibody removal characterization of Her2 (Fig. 2D), expressing also CK and negative to ER and PR. Scale bar 25 μm.