Table 1.

4-plex protocol applied to the MTP stainer.

Step	Reagent	Incubation time min
1	anti-ER AbI	4
2	HRP-AbII	4
3	TSA-AF	2
4	Elution	6
5	anti-CK AbI	2
6	HRP-AbII	2
7	TSA-AF	2
8	Elution	4
9	anti-PR AbI	4
10	HRP-AbII	4
11	TSA-AF	2
12	Elution	6
13	anti-Her2 AbI	2
14	HRP-AbII	2
15	TSA-AF	2
	Total staining time	48 min
	Total staining time with washing steps	1h24 min

TSA-AF indicates a fluorescent-labeled TSA among AF350, AF488, AF546, AF594 and AF647.