Fig. 5.

Genetic, epigenetic, and selection evidence implicating the ZNF613 gene on gestation length. a GWAS signals (point-plot) and differentially methylated regions (DMR, bar plot) around ZNF613 for gestation length, sire calving ease (SCE), body depth (BDE) and cow conception rate (CCR). Sperm-retained nucleosome is also shown below DMRs. b Methylation levels of the shared DMR (the second intron of ZNF613) in the two compared groups across these four traits. c Comparison of signs of variant effects (i.e., b, based on signal-marker GWAS) within ZNF613 between gestation length and 13 dairy traits, for which ZNF613 is also a candidate gene based on our previous fine mapping analyses. +denotes the same direction, and – denotes the opposite direction. d The changes of minor allele frequency (MAF) of the lead variant (chr18:58141989; P = 7.97e-84) within ZNF613 over the years from 1952 to 2012