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. 2019 Mar 14;9:4532. doi: 10.1038/s41598-019-40241-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo anti-tumor efficacy of two antibody blockade both CD47 antibody and CD274 antibody in 4T1 tumor cells. (A) Flow cytometry sorting graphs showing how to distinguish unwanted noise from gated. (B) Schema for two antibodies blockade in immunotherapy. (C–E) The percent of T cells, NK cells and NKT cells in leukocyte since the start of the injection (*P < 0.05, **P < 0.01 and ***P < 0.001). (F–H) The percentage activation of T cells, NK cells and NKT cells after treatment.