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. 2019 Mar 14;9:4557. doi: 10.1038/s41598-019-40923-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of Lef1 expression. At PND10 there was robust Lef1 expression in uterine stromal cells, in particular at the mesometrial pole. In ACD+/− mutants (right panels) Lef1 expression was greatly reduced. Reduced Lef1 expression in mutants was also seen in adults at diestrus, estrus and at E5 post fertilization. During estrus there was Lef 1 expression in GE as well as stroma in WT uterus. Arrows point to uterine glands. Scale bars in microns.