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. 2019 Mar 14;9:4557. doi: 10.1038/s41598-019-40923-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of WT, ACD+/− and ACD+/−WTA11 cells. Cells of all three genotypes were combined for unsupervised clustering analysis. Right panel shows t-SNE plot color coded according to resulting cells types, as labeled. Left panel shows the same t-SNE only color coded according to genotype. Of interest, the two cells types, stromal and myocyte, with strongest expression of the mutated Hox genes show very distinct cell separations based on genotype. For example, the stromal cluster includes ACD+/− cells grouping at the top, WT cells at the bottom, and ACD+/−WTA11 cells in between. Myocytes show a very similar separation. Other cell types, such as endothelial, and lymphocytes, with little expression of the Hox genes mutated, show no such separation based on genotype, arguing against a simple batch effect explanation.