Figure 1.

ABCA1 inhibits pore formation by SLO in an ATPase-dependent manner. (A) HEK293 cells transiently transfected with plasmids encoding GFP, ABCA1-GFP, and ABCA1(MM)-GFP were treated with SLO and DAPI. To make images clearer for counting DAPI-stained and GFP-positive cells, the images were processed (panel at the right end) as described in Methods. (B) DAPI-stained (magenta in processed image) and unstained GFP-positive cells were counted, and proportions were calculated. Average values are shown with S.E. For each samples, images were acquired at five positions in the dish. **P < 0.001 vs. control (GFP).